chromosome walking การใช้

• Thus, sequences not reachable by chromosome walking can be sequenced.
• For genomes in which the regions of locus and is known as chromosome walking.
• It is related to several other tools used for the same purpose, including chromosome walking.
• The end of one of these clones can then be sequenced to yield a new probe and the process repeated in a method called chromosome walking.
• The term " "'chromosome walking "'" is used instead when we know the sequence but don't have a clone of a gene.
• Chromosome walking can be used from the new jump position ( in either direction ) to look for gene-like sequences, or additional jumps can be used to progress further along the chromosome.
• Chromosome jumping is used to bypass regions difficult to clone, such as those containing repetitive DNA, that cannot be easily mapped by chromosome walking, and is useful in moving along a chromosome rapidly in search of a particular gene.
• Two principal methods are used for this : primer walking ( or " chromosome walking " ) which progresses through the entire strand piece by piece, and shotgun sequencing, which is a faster but more complex process that uses random fragments.